Received 10 August 2021, Accepted 23 February 2022, Available online 28 February 2022.
State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan, 430071, China
“Monkey poxviruses (MPXVs) are large DNA viruses with reported genome sizes ranging from 190,083 to 206,372 bp in length. As a member of the Orthopoxvirus genus in the family Poxviridae, MPXV is subdivided into the West African and Congo Basin clades. The latter is more pathogenic and has been reported to infect humans in various parts of the world [reviewed in (Brown and Leggat, 2016)]. Quantitative polymerase chain reaction (qPCR) is the gold standard for the detection of orthopoxvirus (including MPXV). For pan-orthopoxviruses detection, the E9L (DNA polymerase) gene has been shown to be an excellent target for qPCR assays (Kulesh et al., 2004).